Extended Data Fig. 2: Multiplex tetramer staining by mass cytometry.
From: Bystander CD8+ T cells are abundant and phenotypically distinct in human tumour infiltrates
To investigate the antigen specificity of CD8+ TILs, we performed multiplex MHC-tetramer staining as reported previously (see Methods). By using a three-metal coding scheme, we encoded up to 120 different tetramers specific for neoantigens, tumour-associated antigens (TAA) and cancer-unrelated epitopes (peptide list is shown in Supplementary Tables 1–3). Data are from at least 10 independent mass cytometry experiments (See Fig. 2). Representative data from three patients. Each MHC tetramer+ cell population is positive for a unique code, composed of three differently-labelled streptavidin populations.
