Extended Data Fig. 5: PNO1, NOB1 and coordination of the 3′ rRNA end.
From: Visualizing late states of human 40S ribosomal subunit maturation
a, The 3′ end region (box) throughout the maturation process. PNO1 and NOB1 bind similarly in state B–E and R. b, Cartoon representation of eS26 and PNO1 with parts of the pre-18S rRNA at different stages in maturation. h44 is shifted in state C. Displacement of eS26 by PNO1 in state R leads to a partially shifted h44. c, Overall structure of PNO1 with its two KH domains. d, e, Detailed view on the effects of PNO1 binding. Clashing of PNO1 with h28 and the linker region between h44 and h45 leads to a disruption of the base stacking between G1207 and G1837 (d) and A1835 and A1863 (e). f, Residues involved in novel base pairing with their respective electron density in state C. g, Comparison of the PIN domain of human (hs) NOB1 in state C and the NMR structure of Nob1 from Pyrococcus horikoshii (ph; PDB code 2LCQ), with their conserved active site residues highlighted. h, Summary of residues involved in binding of the rRNA 3′ end (asterisk indicates stacking). Electron density of state C surrounding the 3′ end is shown.
