Extended Data Fig. 2: Local resolution, refinement and model statistics.
From: Visualizing late states of human 40S ribosomal subunit maturation
a, Local resolution distribution as estimated by Relion ranging from approximately 3 Å in well-resolved areas to 12 Å in more flexible parts. Colouring according to scale bars. b, Fourier shell correlation (FSC) plot with average resolutions according to the ‘gold standard’ (FSC = 0.143) stated in the legend. c, FSC plot of the state C model against cryo-EM maps as calculated by REFMAC5 (see Supplementary Data 1 for source data). d, Structural comparison between state C and a pre-40S particle from yeast (PDB code 6EML). Assembly factors coloured as in Fig. 1a. e, Cartoon representations of models of RBFs (top) are shown together with their respective density (mid). Volumes are coloured according to local resolution, which ranges from 3 Å in more rigid areas to 9 Å in flexible parts. RRP12, BUD23 and TRMT112 are less resolved, which only permitted placing of dummy helices and rigid body fitting of homology models, respectively. Examples of well resolved areas are depicted below.
