Extended Data Fig. 6: Alignment of CD sequences and ACC–citrate filament interface.
From: Structural basis for regulation of human acetyl-CoA carboxylase
a, The intermolecular interface in ACC–citrate filaments is shown in cartoon representation with the transparent electron microscopy map shown in grey. Local two-fold symmetry is indicated, and domains of the lower dimer are labelled. The map is shown at contour level of 0.0189. b, Close-up of the interface as indicated in a. ACC–citrate is shown in colour as cartoon representation. ScACC is shown as a cartoon in grey and superimposed for one side of the interface. For the other side, an additional surface representation is shown for ACC–citrate. The loop between Nα4 and Nα5 of the four-helix bundle of ACC–citrate CDN domain binds in the cradle formed by Lα2, Lα4 and β1. This loop is substantially shorter in ScACC, demonstrating incompatibility of ScACC with formation of the interface. c, Same depiction as in b, but the surface of the top ACC–citrate dimer is shown and ScACC, shown in grey, is superimposed on the bottom dimer. The extended loop in ScACC between Lα1 and Lα2 is not compatible with filament formation. d, Alignment of 20 ACC CD sequences of metazoan and fungal organisms. Residue numbers according to human ACC are indicated as well as the helices, the loops and the strand labelled in b and c. Darker colour indicates increased conservation. Pairwise identity over all aligned sequences is 61.5%, pairwise identity over metazoan and fungal sequences is 97.0% and 54.0%, respectively. Accession numbers: Homo sapiens, Q13085; Bos taurus, Q9TTS3; Canis lupus familiaris, E2RL01; Capra hircus, XP_017919660; Danio rerio, F1QH12; Drosophila melanogaster, Q7JV23; Felis catus, XP_011287256; Gallus gallus, P11029; Gorilla gorilla gorilla, XP_018881836; Mus musculus, Q5SWU9; Rattus norvegicus, P11497; Sus scrofa, D2D0D8; Aspergillus nidulans, AN6126.2; Candida glabrata, Q6FKK8; Candida albicans, C4YNG3; Chaetomium thermophilum, G0S3L5; Kluveromyces lactis, Q6CL34; Saccharomyces cerevisiae, Q00955; Schizosaccharomyces pombe, P78820; Trichophyton verrucosum, D4DIV5.
