Extended Data Fig. 3: Processing of electron microscopy data for ACC–BRCT filaments and validation.
From: Structural basis for regulation of human acetyl-CoA carboxylase
a, Flowchart of data processing showing a raw micrograph, 2D classes, 3D classes and refinement. b, Overview of map quality for the CDC2, CT and CDN domains. Protein is shown in colour according to scheme in Fig. 1a, with transparent electron microscopy map. Maps are shown at contour level of 0.009. c, FSC curves for masked and unmasked as well high-resolution phase-randomized reconstructions and final corrected FSC curve. Maps coloured according to local resolution, colour scale is provided. Electron microscopy maps are shown at contour level of 0.019 and 0.105 for mask 1 and mask 2, respectively.
