Extended Data Fig. 1: Binding characteristics of scFv.

a, b, scFv 16 does not perturb the interfaces between Gα and Gβ at its binding epitope (a) or the switch II region located ~40 Å away (b). Our structure is coloured by chain, whereas the structure of GDP-bound G_i1 heterotrimer (PDB code 1GP2) is coloured grey. c, In the nucleotide-free state (coloured by subunit), there is a ~7° rotation of Gβγ relative to the Gα_s switch II domain when compared to the GDP-bound form. d, This rotated conformation is similar to that observed in nucleotide-free G_s coupled to the β₂AR (PDB code 3SN6).