Extended Data Fig. 2: Sample preparation for HDX-MS and selected raw data.

a, Representative LC–MS spectrum of the prepared Ub-VS probe (see Methods). Experiment was performed in duplicate. b, Representative LC–MS spectrum of Ub-VS-reacted phospho-parkin. Experiment was performed in duplicate. c, Samples used in HDX-MS analysis. In HDX-MS, non-covalent complexes with phospho-ubiquitin were used. Covalent complexes are indicated with a dash and non-covalent complexes by a colon. This is representative of at least three independent experiments; for gel source data, see Supplementary Fig. 1. d, Relative deuterium uptake (in Da) is shown for exemplary selected peptides across the parkin molecule, over the timecourse of the experiment. Each point for the technical replicate experiments is shown. Data points were taken at identical time points, but are offset on the x axis for clarity.