Extended Data Fig. 5: Most TfR1 residues that interact with PvRBP2b are specific to human TfR1.

a, Schematic representation of TfR1 sequence coloured according to domain. Apical domain, green; protease-like domain, red; helical domain, yellow. Domain boundaries are indicated above the schematic. The most important residues forming either hydrogen bonds or salt bridges with PvRBP2b are indicated below with black arrows. b, Comparison between Machupo virus GP1 and PvRBP2b binding sites. Human TfR1 is shown in the centre in surface representation and coloured according to the domain organization. The residues that interact with PvRBP2b are shown in violet and those that interact with Machupo virus GP1 are dark blue. Residues overlapping the two sites are shown in magenta. The surrounding panels show fragments of the alignment between TfR1 sequences from different species (human, mouse, rat, hamster, cat and dog). Residues are coloured according to their chemical properties (acidic, red; basic, blue; polar, magenta; cysteine, yellow; hydrophobic, grey scale with intensity proportional to hydrophobicity). The fragments of the alignment corresponding to the apical and protease-like domains of TfR1 are additionally shaded in green and red, respectively. The residues interacting with PvRBP2b are indicated with stars above the alignment, which are coloured according to the type of interaction (hydrogen bonds, green; salt bridges, red; others, empty stars). The Machupo virus GP1-interaction site is indicated with a dark blue line. The position of the G217 deletion and the G142S polymorphism are indicated with black arrows above the alignment. c, Analytical SEC results showing PvRBP2b(161–1454) interaction with mouse Tf and TfR1. For gel source data, see Supplementary Fig. 1. Top, elution profiles for the separate components and for the combined components. Bottom, SDS–PAGE of SEC fractions. Left, human Tf and human TfR1; middle left, mouse Tf and human TfR1; middle right, human Tf and mouse TfR1; right, mouse Tf and mouse TfR1. The same representative SDS–PAGE gel is used for PvRBP2b protein SEC analyses. All analytical SEC experiments were performed once.