Extended Data Fig. 5: Additional whole mount immunofluorescence of marker genes.
From: Single-cell analysis of early progenitor cells that build coronary arteries
a, CX40 whole-mount immunohistochemistry in late gestation hearts (E17.5). CX40 is expressed only in cells lining large arteries and arterioles (overlapping blue and green signal). Low level, non-arterial signal is in myocardial cells. b, rPCA plots from E12.5 and E14.5 with accompanying whole-mount immunofluorescence in E13.5 hearts. VWF is enriched in the SV; while APLN–nlacZ signal and DACH1 are present throughout the coronary plexus. CXCR4, ACKR3–GFP, and CXCL12–DsRed are enriched in the pre-artery and artery areas of rPCA plots and are interspersed within the intramyocardial coronary plexus. n = 415 cells (left, E12.5); n = 347 cells (right, E14.5). Scale bars, 100 μm.
