Extended Data Fig. 10: Gene expression curves in E14.5 control and Coup-tf2OE cells.
From: Single-cell analysis of early progenitor cells that build coronary arteries
a, FACS plots of the GFP-marked cells from control and Coup-tf2OE hearts that were processed for scRNA-seq. b, Criteria for identifying Coup-tf2OE cells was >1 read of the flag and myc sequences included in the transgene. c, Comparing the number of flag and myc reads in control and Coup-tf2OE hearts confirms the specificity of this parameter for transgenic cells. Control: n = 409 cells. COUP-TF2OE: n = 714 cells. The centre line corresponds to the median; the upper and lower hinges correspond to the first and third quartile, respectively; the whiskers extend to the largest value or to 1.5 × IQR (inter-quartile range, or distance between quartiles), whichever is smaller. d, Expression of genes from the indicated categories along the vein–CV plexus–arterial axis. The x-axis has individual cells organized as shown in Fig. 5b. Lines are LOESS curves of gene expression and raw data points are shown as dots. Shaded region represents the 95% confidence interval of the LOESS curve. e, Hypoplastic coronary vasculature with heterozygous deletion of Coup-tf2 in endothelial cells. Scale bars, 100 μm.
