Extended Data Fig. 4: Novel artery markers identified in scRNA-seq data.
From: Single-cell analysis of early progenitor cells that build coronary arteries
a, E12.5, E14.5, and adult coronary cell rPCA plots with genes highly enriched or specific to the arterial area during development. Each dot is an individual cell, and gene expression levels are indicated by the colour spectrum as shown in Fig. 1d, which reflects log10CPM. Genes in bold red are also enriched in adult artery cells. b, Fluorescence in situ hybridization (RNAscope) for Slc45a4, which is expressed (arrowheads) in vessels positive for the arterial marker Cx40, but not in Cx40-negative capillaries (arrows). c, Slc45a4 expression in pre-artery cells derived from the SV lineage (ApjCreER lineage-labelled; arrowheads). d, Genes enriched in, but not specific to, arterial cells at E12.5 and E14.5. Genes in bold red are arterial specific in both the developing and adult heart. In a and d: for PCA plots, n = 415 cells (top, E12.5); n = 347 cells (middle, E14.5); n = 445 cells (bottom, adult). For bar graphs E12.5, Art, n = 20 cells; CV, n = 277 cells; SV, n = 118 cells; E14.5, Art, n = 70 cells; CV, n = 454 cells; SV, n = 144 cells. Centre and error bars are mean ± s.e.m. of log CPM expression values. Dots represent individual cells. Scale bars, 100 μm.
