Extended Data Fig. 8: IgM–TLR9 PLA identifies ABC samples with chronic active BCR signalling in tissue microarrays.
From: A multiprotein supercomplex controlling oncogenic signalling in lymphoma
a, IgM–TLR9 PLA was performed on a formalin-fixed, paraffin-embedded (FFPE) tissue microarray of lymphoma cell lines. PLA puncta were quantified and plotted as the absolute number of spots per cell from at least 2 images of 1 representative experiment from 3 independent tissue microarray replicates. Box plots represent median and 25–75% of data, whiskers display range. Cell lines are divided by putative lymphoma subtype for presentation. BL, Burkitt lymphoma; BPDC, blastic plasmacytoid dendritic cell neoplasm; HL, Hodgkin lymphoma; MZL, marginal zone lymphoma; PMBL, primary mediastinal B cell lymphoma; WM, Waldenström’s macroglobulinemia. b, Representative confocal fluorescent image from three independent biological samples of a germinal centre from a reactive lymph node. IgM–TLR9 PLA is shown in red; CD20 is in green; CD138 is in white; and DAPI is in blue.
