Extended Data Fig. 9: Waldenström’s macroglobulinaemia can utilize the My-T-BCR supercomplex.
From: A multiprotein supercomplex controlling oncogenic signalling in lymphoma
a, shRNA-mediated toxicity of indicated genes in two Waldenström’s macroglobulinaemia cell lines (RPCI-WM1 and MWCL-1). Control (SC4), CD79A, TLR9 or MYD88 shRNAs were expressed in tandem with GFP and the relative level of GFP was followed over time by FACS. Data are mean and s.e.m. of independent biological experiments; see ‘statistics and reproducibility’ section. b, Confocal images from one of two representative biologically independent experiments of the PLA reaction between IgM and TLR9 (red puncta). Cells were counterstained with DAPI (blue) and WGA (green). Scale bars, 10 μm. c, Normalized quantification (PLA score) of IgM–TLR9. Data were quantified from at least 28 cells per condition. Box plots represent median and 25–75% of data, whiskers display range.
