Extended Data Fig. 4: TLR9 overexpression and association with the BCR are features of ABC DLBCL.
From: A multiprotein supercomplex controlling oncogenic signalling in lymphoma
a, Gene expression values (log2 FPKM) values of TLR9 associated genes are shown by DLBCL subtype, ABC in blue (n = 294), GCB in orange (n = 164) and unclassified (Unc) in grey (n = 115). Gene expression data were correlated with DNA copy number and linear regression calculated for ABC samples. *P < 0.05, ***P < 0.001, linear regression (left); *P < 0.05, ***P < 0.0001, one-way ANOVA and Tukey’s post test (right). b, Amplification of the UNC93B1 and CNPY3 loci (black lines, below chromosome ideogram). Minimal shared amplified regions in ABC DLBCL biopsies are bracketed and genes displayed below. c, The essential TLR9 interactome in TMD8. TLR9–BioID2 interactome determined by SILAC-based mass spectrometry (y axis) plotted by the CRISPR screen score (CSS, x axis). Bait (TLR9) is labelled in blue. Essential interactors are labelled in red, those shared with HBL1 (Fig. 2c) are labelled in dark red. d, Venn diagram of the overlap of TLR9–BioID2 interactors identified by SILAC-based mass spectrometry in experiments performed in TMD8 and HBL1 ABC lines. The 47 overlapping proteins are listed. e, The enrichment of 47 overlapping TLR9–BioID2 proximal proteins is shown (top) relative to their CSS (bottom). Gene names labelled in red are enriched and toxic to both HBL1 and TMD8. See ‘statistics and reproducibility’ section.
