Extended Data Fig. 4: Growth curve of S. cerevisiae ∆ILV3 expressing AstD and AteDHAD. | Nature

Extended Data Fig. 4: Growth curve of S. cerevisiae ∆ILV3 expressing AstD and AteDHAD.

From: Resistance-gene-directed discovery of a natural-product herbicide with a new mode of action

Extended Data Fig. 4: Growth curve of S. cerevisiae ∆ILV3 expressing AstD and AteDHAD.The alternative text for this image may have been generated using AI.

a–d, The genome copy of DHAD encoded by ILV3 was first deleted from S. cerevisiae strain DHY ΔURA3 to give UB02. UB02 was then either chemically complemented by growth on ILV (leucine, isoleucine and valine)-containing medium or genetically by expressing of AteDHAD (fungal housekeeping DHAD from A. terreus, fDHAD) or AstD episomally (TY06 or TY07, respectively). The empty vector pXP318 was also transformed into UB02 to generate a control strain TY05. Cell growth (optical density) under different conditions was plotted as a function of time. Data are mean ± s.d. from three biologically independent experiments. a, Growth curve in ILV dropout medium with no aspterric acid. b, Growth curve in ILV dropout medium with 125 μM aspterric acid. c, Growth curve in ILV supplemented medium. d, Growth curve in ILV supplemented medium with 250 μM aspterric acid.

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