Extended Data Fig. 6: Localization of CST and Polα to DSBs.
From: 53BP1–RIF1–shieldin counteracts DSB resection through CST- and Polα-dependent fill-in
a, Quantification of HA–STN1 localization to DSBs induced by FOKI, as in Fig. 3e. Mean (centre bars) and s.d. (error bars) from 4–6 independent experiments, with >80 induced nuclei for each condition in each experiment. b, Immunofluorescence for endogenous Polα in FOKI–LacI U2OS cells in S phase and after RO3306 treatment (G2). Dotted lines denote the outline of the nucleus. Representative of two experiments. c, Examples of HA–STN1 and Polα localization at DSBs induced by FOKI in G2-arrested FOKI–LacI U2OS cells (as in Fig. 3f). Representative of three experiments. d, Quantification of co-localization of Polα with DSBs induced by FOKI (as in Fig. 3f). Mean (centre bars) and s.d. (error bars) from three independent experiments, with >80 induced nuclei for each condition in each experiment. **P < 0.01, ***P < 0.001, ****P < 0.0001, two-tailed Welch’s t-test.
