Extended Data Fig. 5: CST interacts with shieldin.
From: 53BP1–RIF1–shieldin counteracts DSB resection through CST- and Polα-dependent fill-in
a, Immunoprecipitation of individual mouse CST subunits or the three subunit complex (each subunit bearing a Myc tag) with Flag-tagged mouse SHLD1, co-expressed in 293T cells. Flag-tagged POT1B and POT1A serve as positive and negative controls for CST binding, respectively. Representative of two experiments. b, Two-hybrid analysis of CST-shieldin interaction. Yeast cultures were grown overnight in synthetic complete medium that lacked tryptophan and leucine, to a density of 5 × 107 cells per millilitre. Serial tenfold dilutions were generated and 4 μl of each dilution was spotted on synthetic complete medium that lacked the nutrients tryptophan, leucine, adenine and histidine, and contained 3-aminotriazole (3-AT), as indicated. Plates were then incubated for 5 days at 30 °C before imaging. Representative of three experiments.
