Extended Data Fig. 7: Detection of nanobody coupling to β1AR.
From: PtdIns(4,5)P2 stabilizes active states of GPCRs and enhances selectivity of G-protein coupling
Peaks in the mass spectrum assigned to Nb6B9 binding to β1AR to form an equimolar β1AR–Nb6B9 complex are highlighted in orange, and demonstrate complete complex formation, implying that nanobody has a higher affinity than mini-Gs for β1AR. n = 3 independent experiments.
