Extended Data Fig. 3: 13C-isotopologue labelling of glucose and TCA cycle metabolites in mouse BAT following intravenous [13C]glucose at 29 °C or 4 °C.
From: Accumulation of succinate controls activation of adipose tissue thermogenesis
![Extended Data Fig. 3: 13C-isotopologue labelling of glucose and TCA cycle metabolites in mouse BAT following intravenous [13C]glucose at 29 °C or 4 °C.](http://media.springernature.com/full/springer-static/esm/art%3A10.1038%2Fs41586-018-0353-2/MediaObjects/41586_2018_353_Fig7_ESM.jpg?as=webp)
a, Potential inputs to succinate-directed flux by conventional BAT metabolism and 13C-metabolite labelling strategy. Mice were administered [U-13C]glucose (b–h) or [U-13C]palmitic acid (i–m) intravenously as a bolus at 29 °C or 4 °C followed by BAT harvesting and snap freezing for LC–MS analysis at indicated time points. b, Proportional isotopic labelling of BAT glucose. c, d, Proportional isotopic labelling profile of glycolytic metabolites 3-phosphoglycerate (c) and lactate (d) in mouse BAT. e–h, Proportional isotopic labelling profile of TCA cycle metabolites citrate (e), succinate (f), fumarate (g), and malate (h) in mouse BAT. i, Proportional isotopic labelling of BAT palmitate. j–m, Proportional isotopic labelling profile of TCA cycle metabolites citrate (j), succinate (k), fumarate (l), and malate (m) in BAT (n = 5). Missing values or N.D. indicate value not determined. Data are mean ± s.e.m. of biologically independent samples.
