Extended Data Fig. 5: Representative OCR experiments for brown adipocytes and other cell types with or without acute addition of succinate and related substrates. | Nature

Extended Data Fig. 5: Representative OCR experiments for brown adipocytes and other cell types with or without acute addition of succinate and related substrates.

From: Accumulation of succinate controls activation of adipose tissue thermogenesis

Extended Data Fig. 5: Representative OCR experiments for brown adipocytes and other cell types with or without acute addition of succinate and related substrates.The alternative text for this image may have been generated using AI.

a, Representative OCR trace monitoring effect of acute addition of succinate or noradrenaline (NE) in the De2.3 immortalized brown adipocyte cell line32 (vehicle, n = 7; succinate, n = 6; NE, n = 7). b, Representative OCR trace monitoring dose-dependent effect of acute addition of succinate in the De2.3 immortalized brown adipocyte cell line (vehicle, n = 7; 1 mM succinate, n = 6; 5 mM succinate, n = 7). c, Representative OCR trace monitoring dose-dependent effect of acute addition of succinate in various cell types (subcutaneous white adipocytes: vehicle, n = 6; 1 mM, n = 6; 5 mM, n = 5; myoblasts: vehicle, n = 7; 1 mM, n = 6; 5 mM, n = 7; HEK: vehicle, n = 7; 1 mM, n = 6; 5 mM, n = 7; hepatocytes: vehicle, n = 7; 1 mM, n = 6; 5 mM, n = 7; osteocytes: vehicle, n = 7; 1 mM, n = 6; 5 mM, n = 7; A549 lung: vehicle, n = 7; 1 mM, n = 6; 5 mM, n = 7; brown pre-adipocytes: vehicle, n = 7; 1 mM, n = 6; 5 mM, n = 7). d, Effect of acute addition of succinate on cellular OCR in human brown adipocytes (basal, n = 6; 1 mM, n = 6; 5 mM, n = 7). e, Representative OCR trace monitoring dose-dependent effect of acute addition of succinate in human immortalized brown adipocytes (vehicle, n = 7; 1 mM succinate, n = 6; 5 mM succinate, n = 7). f, Inhibition of succinate-stimulated OCR in brown adipocytes by DTNB. g, Representative OCR experiment (n = 7; 0.1 mM DTNB, n = 6). h, i, Inhibition of succinate-stimulated OCR in brown adipocytes by DIDS. n = 12, except 1 mM DIDS, n = 8). j, k, Inhibition of succinate-stimulated OCR in brown adipocytes by treatment with the Na+/K+ ATPase inhibitor ouabain (n = 5). Effects on respiration were determined by acute addition of oligomycin (oli) to determine leak respiration, 2,4-dinitrophenol (DNP) to determine chemically uncoupled maximal respiration, or rotenone plus antimycin (r/a) to determine non-mitochondrial respiration. In all cases basal respiration in these cells is measured in the presence of 1 mM pyruvate. Two-sided t-test (d); two-way ANOVA (f, h, j); data are mean ± s.e.m. of biologically independent samples.

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