Extended Data Fig. 5: Effects of IRF1 on priming, mitochondrial damage and non-canonical inflammasome activation.
From: New mitochondrial DNA synthesis enables NLRP3 inflammasome activation
a, Immunoblot analysis of NLRP3, ASC, pro-caspase-1, pro-IL-1β, NEK7 and IRF1 in the lysates of wild-type and Irf1−/− BMDMs before and after LPS stimulation. Data are typical of three separate experiments. b, Amounts of TNF in culture supernatants of LPS-primed wild-type and Irf1−/− BMDMs that were stimulated with the indicated inflammasome activators. Data are mean ± s.d. (n = 3 biological replicates). c, Percentages of Ψm preservation were measured by TMRM fluorescence in LPS-primed wild-type and Irf1−/− BMDMs that were stimulated with indicated NLRP3 activators. Data are mean ± s.d. (n = 3 biological replicates). d, Relative amounts of mtROS were measured by MitoSOX fluorescence in LPS-primed wild-type and Irf1−/− BMDMs that were stimulated with indicated NLRP3 activators. Data are mean ± s.d. (n = 3 biological replicates). e, Immunoblot analysis of caspase-11 and IRF1 in lysates from wild-type and Irf1−/− BMDMs before and after LPS stimulation. Results are typical of three independent experiments. f, Amounts of IL-1β in culture supernatants of LPS-primed wild-type and Irf1−/− BMDMs that were further transfected with FuGENE-complexed LPS. Data are mean ± s.d. (n = 3 biological replicates).
