Extended Data Fig. 6: IRF1 mediates LPS-induced CMPK2 expression.
From: New mitochondrial DNA synthesis enables NLRP3 inflammasome activation
a, Relative amounts of Cmpk2 mRNA in wild-type and Irf1−/− BMDMs before and after LPS stimulation. Data are mean ± s.d. (n = 3 biological replicates per time point). b, Immunoblot analysis of CMPK2, VDAC and tubulin in mitochondrial and cytosolic fractions of wild-type BMDMs after LPS stimulation. Results are typical of three separate experiments. c, Chromatin immunoprecipitation analysis of IRF1 recruitment to the Cmpk2 promoter. Data are mean ± s.d. (n = 4 biological replicates for wild-type and Irf1−/− groups; n = 5 and 6 biological replicates for wild-type + LPS and Irf1−/− + LPS groups, respectively). Cxcl10, a known IRF1-target gene, was included as a positive control. d, Relative mRNA amounts of dGK (also known as Dguok), Tk2, Ak2, Nme4 and Polg in wild-type BMDMs before and after 6 h LPS stimulation. Data are mean ± s.d. (n = 3 biological replicates). e, Immunoblot analysis of the enzymes encoded by the genes in d in the lysates of wild-type BMDMs before and after LPS stimulation. Results are typical of three independent experiments. f, Immunoblot analysis of CMPK2 and NME4 in shCtrl- or specific shRNA-transduced BMDMs. Data are typical of three independent experiments. *P < 0.05; **P < 0.01; two-sided unpaired t-test.
