Extended Data Fig. 1: Role of TLR5 and flagellin expression in the pathogenesis of neonatal Salmonella infection.
From: Neonatal selection by Toll-like receptor 5 influences long-term gut microbiota composition
a, b, Intestinal colonization and organ dissemination evaluated by serial dilution and plating two (a) or three (b) days after infection of 1-day-old wild-type (WT, filled circles, n = 5) or Tlr5-deficient mice (Tlr5, open circles, n = 4 or n = 5, respectively) with approximately 102 CFU S. Typhimurium. Results from one litter at each time point. c, Intestinal colonization and organ dissemination evaluated by serial dilution and plating in small intestine (n = 6 and 11), colon (n = 7 and 11), liver (n = 10 and 12), spleen (n = 10 and 12) and lung (n = 10 and 12) 4 days after infection of 1-day-old WT (filled symbols) mice with approximately 102 WT S. Typhimurium (circles, two litters) or ΔfljB/fliC mutant S. Typhimurium (triangles, three litters). d, Intestinal colonization and organ dissemination evaluated by serial dilution and plating in small intestine (n = 6 and 4), colon (n = 9 and 4), liver (n = 9 and 4), spleen (n = 9 and 4) and lung (n = 9 and 4) 4 days after infection of 1-day-old Tlr5-deficient (open symbols) mice with approximately 102 WT S. Typhimurium (circles, 1 litter) or ΔfljB/fliC mutant S. Typhimurium (triangles, 1 litter). Data in a–d represent the median (two-sided Mann–Whitney test). e, Survival of 1-day-old WT mice infected orally with wild-type (wt, dashed line, n = 20, three litters) or isogenic flagellin-deficient (ΔfljB/fliC, solid line, n = 22, three litters) S. Typhimurium. f, Cxcl2 mRNA expression analysed by quantitative RT–PCR in intestinal epithelial cells (IECs) isolated 4 days after infection of 1-day-old wild-type mice (WT, filled symbols, n = 13 and 11 from each three litters) or Tlr5-deficient mice (Tlr5, open symbols, n = 6 and 4 from each two litters) with wild-type S. Typhimurium (wt, circles) or an isogenic flagellin mutant (ΔfljB/fliC, triangles). Values were normalized to the expression of the housekeeping gene Hprt. Data represent the median (*P < 0.05; **P < 0.01, two-sided Mann–Whitney test).
