Extended Data Fig. 4: Neonatal but not adult epithelial Reg3g mRNA expression is influenced by TLR5 expression.
From: Neonatal selection by Toll-like receptor 5 influences long-term gut microbiota composition
IECs from non-infected wild-type (WT, red) and Tlr5-deficient (Tlr5, blue) mice were collected at 1 (n = 9 from three litters and n = 7 from three litters), 4 (n = 7 from two litters and n = 10 from two litters), 10 (n = 9 from one litter and n = 11 from two litters) and 21 days (n = 9 from one litter and n = 6 from one litter) after birth and Reg3g mRNA expression was analysed by qRT–PCR. Values were normalized to the expression of the housekeeping gene Hprt. Data represent the median (*P < 0.05; **P < 0.01; ***P < 0.001; two-sided Mann–Whitney U-test).
