Extended Data Fig. 7: Immunogold negative-stain EM analysis of additional Pick’s disease cases.
From: Structures of filaments from Pick’s disease reveal a novel tau protein fold
a, Representative immunogold negative-stain electron microscopy with antibodies BR133 (tau N terminus), BR136 (tau R1), anti-4R (tau R2), BR135 (tau R3), TauC4 (tau R4) and BR134 (tau C terminus) of NPFs and WPFs extracted from the frontotemporal cortex of patient 4. Scale bars, 100 nm. Similar results were obtained with tau filaments extracted from the frontotemporal cortices of eight additional patients with Pick’s disease. b, Table summarizing results from a. Results for patient 4 (shown in a and used for cryo-EM) are highlighted in yellow. See Extended Data Table 1 for details of the patients with Pick’s disease. Tick marks indicate antibody decoration of filaments, and crosses indicate that the antibodies did not decorate filaments. NPFs and WPFs were decorated by the antibodies against the N and C termini of tau, but not by the tau repeat-specific antibodies.
