Extended Data Fig. 3: WPF structure.
From: Structures of filaments from Pick’s disease reveal a novel tau protein fold
a, Fourier shell correlation curves between two independently refined half-maps. b, Local resolution estimates for the WPF reconstruction. c, WPF density at high (light grey) and low (dark grey) threshold with densities for two NPFs overlaid (yellow and blue). The atomic models fitted to the NPF densities in the region of the protofilament interface are shown in the boxed out area. d, Cryo-EM images showing WPFs from patient 4 (false coloured red), in which segments from one of the protofilaments have been lost. Scale bar, 500 Å. e, Negative-stain EM images of WPFs from patient 4 after incubation in 100 mM dithiothreitol for 20 h. Scale bar, 500 Å.
