Extended Data Fig. 6: Proteome wide bioinformatics analysis of Ssb1 interplay with putative onset of cotranslational assembly interactions.

a, Metagene analysis of Ssb1–GFP interaction profiles with the nascent chains of 116 yeast proteins identified as putative cotranslationally assembling subunits (putative assembly identification algorithm and parameters are detailed in the Supplementary Information). The dark grey line indicates Ssb interaction profiles⁴, aligned to the subunits putative onset of cotranslational subunit association positions depicted as 0 (onset position alignment). A zoomed-in view of the nascent-chain segments at assembly onset position ±75 amino acids is shown. The orange line indicates Ssb binding profiles for nascent chains aligned to random positions along the ORFs. Data are from two biologically independent experiments. The area between replicates is shaded, indicating the degree of experimental variation. There is no correlation detected between the random and onset position alignment (Pearson correlation r² = 0.2911), thus Ssb depletion at positions of onset is significant. b, Average Kyte–Doolittle hydrophobicity plot (7-amino-acid window) of the 116 nascent-chain segments. A zoomed-in view of the nascent-chain segments at assembly onset position ±75 amino acids is shown, as in a.