Extended Data Fig. 7: Cotranslational interactions networks of FAS β, Cpa2 and PFK β metabolic enzymes subunits, analysed by SeRP.
From: Cotranslational assembly of protein complexes in eukaryotes revealed by ribosome profiling
a, Fatty acid synthesis metabolic pathway: nascent Faa1 is not engaged by C-terminally-tagged FAS complex β subunit, whereas nascent Acc1 shows a transient interaction, crossing the twofold enrichment threshold, at position approximately 250 codons/amino acids (indicated by an arrow). b, Arginine biosynthetic pathway: nascent Arg4 (argininosuccinate lyase) is not engaged by C-terminally-tagged Cpa2 subunit, whereas nascent Arg1 shows several transient interactions crossing the twofold enrichment threshold, at positions indicated by arrows. c, Glycolysis pathway: nascent Fba1 (fructose 1,6-bisphosphate aldolase) is not engaged by C-terminally tagged PFK complex β subunit, whereas Pyc2 (pyruvate carboxylase isoform) shows several transient interactions crossing the twofold enrichment threshold, at positions indicated by arrows. a–c, Data are from two biologically independent experiments. The area between replicates is shaded, indicating the degree of experimental variation.
