Extended Data Fig. 6: Mutational screen of tyrosine residues present in the cytoplasmic domain of BAK1.
From: Phosphocode-dependent functional dichotomy of a common co-receptor in plant signalling
a, Total ROS production after treatment of bak1-4 mesophyll protoplasts with 100 nM elf18 for 30 min. The RLU values are normalized relative to protoplasts expressing wild-type (WT) BAK1 and are expressed as aligned dot blots showing mean ± s.e.m. n = 4 independent biological experiments. b, Total ROS production of bak1-4 mesophyll protoplasts that transiently express the indicated BAK1 mutants, after treatment with 100 nM elf18 for 60 min. n = 8 biologically independent suspensions. Measurements are plotted as box plots displaying the first and third quartiles, split by the median; whiskers extend to a maximum of 1.5× interquartile range beyond the box. Individual data points are shown as circles. c, Western blot with anti-BAK1 antibodies. For blot source data, see Supplementary Fig. 1. In a and b, statistical analysis was performed using a one-way ANOVA with Dunnett’s post hoc test compared to the wild-type control. For b and c, experiments were repeated twice with similar results.
