Extended Data Fig. 2: Individual or double BAK1(S602A), BAK1(T603A) or BAK1(S604A) mutations do not affect elf18-induced ROS production.
From: Phosphocode-dependent functional dichotomy of a common co-receptor in plant signalling
a, e, Total ROS production of bak1-4 mesophyll protoplasts transiently expressing the indicated BAK1 mutants, after treatment with 100 nM elf18 for 60 min. n = 12 biological independent suspensions. b, d, f, Western blots with anti-BAK1 antibodies. For blot source data, see Supplementary Fig. 1. c, Total ROS production after treatment of leaf discs with 100 nM flg22 for 40 min. Individual data points are shown as circles. n = 8 biologically independent leaf discs. In a, c and e, measurements are plotted as box plots displaying the first and third quartiles, split by the median; whiskers extend to a maximum of 1.5× interquartile range beyond the box. Outliers are indicated as black dots. Statistical analysis was performed using a one-way ANOVA with Dunnett’s post hoc test compared to wild-type BAK1. For a–f, experiments were repeated at least three times.
