Extended Data Fig. 4: Expression of the BAK1(AAA) and BAK1(S612A) variants restores the semi-dwarf rosette phenotype of bak1-4 and bak1-4 bri1-301.
From: Phosphocode-dependent functional dichotomy of a common co-receptor in plant signalling
a, b, Representative images of rosettes from four- to five-week-old plants. c, d, Left, representative images of rosettes from five- to seven-week-old plants. Right, western blots with anti-BAK1 antibodies. Blots were stained with CBB for loading control. Experiments were repeated at least twice with similar results. Control plants used in b have been previously pictured30. e, BES1 dephosphorylation 60 min after treatment of ten-day-old seedlings with the indicated concentrations of epi-BL (µM), as shown by western-blot analysis using anti-BES1 antibodies. Blots were probed with anti-BAK1 antibodies, and subsequently stained with CBB as loading controls. For a–e, experiments were repeated at least three times. For blot source data, see Supplementary Fig. 1.
