Extended Data Fig. 3: Doublet composition of the sorted CD4⁺CD32^high T cells.

Sorted B cells and CD4⁺CD32^neg, CD4⁺CD32^int and CD4+CD32^high T cells from an HIV-1⁺, ART-suppressed participant were analysed using an Amnis imaging cytometer. Singlets and doublets were quantified using the aspect ratio and nuclear staining. a, The proportion of total singlet and doublet events among total nucleated cells detected on the Amnis cytometer in each sorted population was determined, and is shown as individual composite bar graphs for two patients (G07W1610 and OM5365). b, A composite bar graph of the proportion of conjugates, doublets and trogocytotic events that comprised the sorted CD4⁺CD32^high population (n = 2).