Extended Data Fig. 1: Cell sorting strategy.

a, Sorting of stem and progenitor cells. Human bone marrow (BM) and peripheral blood (PB) mononuclear cells (time point 1) were stained with anti-CD34, anti-CD38, anti-CD45RA, anti-CD90, anti-CD10 and anti-CD135 antibodies. After exclusion of debris and doublets, gatings on CD34, CD38 and CD90 were used to separate CD34⁺CD38⁻CD90⁺CD45RA⁻ HSCs. The CD34⁺CD38⁺ compartment was gated for CD10⁻ cells before gating on CD135 (also known as FLT3) and CD45RA to separate progenitor compartments: CD135⁺CD45RA⁻ common myeloid progenitor (CMPs), CD135⁺CD45RA⁺ granulocyte–macrophage progenitors (GMPs) and CD135⁻CD45RA⁻ megakaryocyte–erythrocyte progenitors (MEPs). b, Sorting of B and T lymphocytes. Peripheral blood mononuclear cells (time point, after 4 months) were stained with anti-CD4, anti-CD8 and anti-CD19 antibodies. After exclusion of debris and doublets, the CD4⁺CD8⁺CD19⁻ gate was used to isolate T cells, while the CD4⁻CD8⁻CD19⁺ gate was used to isolate B cells. n = 20,000 events.