Extended Data Fig. 2: Bio-distribution studies of adenovirus-serotype 5 in mice.
From: In vivo CRISPR editing with no detectable genome-wide off-target mutations
a, Schematic of integrated reporter construct in R26R mice used to assess delivery of Cre recombinase using adenovirus serotype 5 vector. Cre-mediated excision of a loxP-flanked transcriptional stop signal upstream of a lacZ gene results in expression of β-galactosidase enzyme. β-Galactosidase expression can be quantified by staining dissected tissues with X-gal, a compound that turns blue when cleaved by this enzyme. b, Quantification of β-galactosidase expression in sections of various dissected organs from n = 2 biologically independent R26R mice intravenously injected with adenovirus serotype 5 vector encoding Cre. Matched organs sections from a R26R mouse intravenously injected with an adenovirus serotype 5 vector encoding GFP were used to determine background staining levels and serve as a negative control. Matched organ sections from Z/EG mice that constitutively express lacZ (β-galactosidase) and intravenously injected with PBS (rather than adenovirus) were used to provide positive staining controls. All mice were evaluated one week after adenovirus or PBS injection. The experiment was performed once.
