Extended Data Fig. 3: Breeding strategy for generating experimental mice containing human PCSK9 open reading frame knocked into the Rosa26 locus. | Nature

Extended Data Fig. 3: Breeding strategy for generating experimental mice containing human PCSK9 open reading frame knocked into the Rosa26 locus.

From: In vivo CRISPR editing with no detectable genome-wide off-target mutations

Extended Data Fig. 3: Breeding strategy for generating experimental mice containing human PCSK9 open reading frame knocked into the Rosa26 locus.

C57BL/6N-derived mouse line containing a single copy of the human PCSK9 open reading frame knocked into the Rosa26 locus (C57BL/6N hPCSK9KI+/−) are used for breeding with C57BL/6N mice. Offspring yielded experimental animals that are C57BL/6N hPCSK9KI+/− (referred to as knock-in) and C57BL/6N hPCSK9KI−/− (referred to as wild type) males.

Back to article page