Extended Data Fig. 4: Scatter plot of CIRCLE-seq read counts for sites identified with gP–Cas9 on genomic DNA from n = 1 wild-type and n = 1 knock-in mice. | Nature

Extended Data Fig. 4: Scatter plot of CIRCLE-seq read counts for sites identified with gP–Cas9 on genomic DNA from n = 1 wild-type and n = 1 knock-in mice.

From: In vivo CRISPR editing with no detectable genome-wide off-target mutations

Extended Data Fig. 4: Scatter plot of CIRCLE-seq read counts for sites identified with gP–Cas9 on genomic DNA from n = 1 wild-type and n = 1 knock-in mice.

Read counts are shown on a logarithmic scale and colours indicate the number of mismatches in each off-target site relative to the on-target site. Sites shown as triangles were chosen for targeted amplicon sequencing. The correlation r2 value obtained using all values in the scatter plot is shown in the upper left-hand corner and was obtained using a linear regression.

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