Extended Data Fig. 8: Effect of alanine mutagenesis of CLR or RAMP1 on CGRP potency in cAMP accumulation assays.
From: Cryo-EM structure of the active, Gs-protein complexed, human CGRP receptor
a, ECD alanine mutations. b, CLR core alanine mutations. Residues that have been mutated are displayed in x-stick format. Mutated residues with no effect on signalling are coloured off-white. Residues that have significantly altered CGRP signalling12,23,28,30,31,32,34,37,38 are also highlighted in transparent CPK representation, coloured according to magnitude of effect. Yellow, <10 fold; dark orange, 10–100 fold; red, 100–1,000 fold; black, >1,000 fold. The backbones of CLR and RAMP (solid lines) are displayed in transparent, off-white coloured ribbon. The CGRP peptide (dashed lines) is represented in x-stick format with carbon atoms in dark red and polar atoms coloured in red or blue.
