Extended Data Fig. 3: Single-turnover kinetic analysis of cA4 degradation by Sso2081 and Sso1393.
From: Ring nucleases deactivate type III CRISPR ribonucleases by degrading cyclic oligoadenylate
a, b, Representative phosphorimages of PAGE, analysing the reaction of radioactively labelled cA4 with 2 μM Sso2081 (a) or 2 μM Sso1393 (b) at 60 °C over time. Experiments were carried out in triplicate, quantified by phosphorimaging and the fraction of cA4 cleaved is plotted in Fig. 3b.
