Extended Data Table 1 Confirmed genes misregulated in OHCs lacking INSM1

From: Trans-differentiation of outer hair cells into inner hair cells in the absence of INSM1

  1. Differential expression of IHCs with respect to OHCs and of OHCs without INSM1 (from Insm1GFP.Cre/– mice, referred to as KO) with respect to OHCs with INSM1 (from Insm1GFP.Cre/+, referred to as Het). Differential expression between KO and Het OHCs estimated by RNA-seq was confirmed by TaqMan RT–qPCR (n = 5 pools of OHCs per genotype for Tbx2, Nhs, Lrrn1, Brip1 and Rin3; n = 4 for all other genes). P values are for one-tailed t-tests on RT–qPCR values. All 22 genes increase their expression in OHCs lacking INSM1. Of these, all except Sez6l are normally preferentially expressed in IHCs. Note that for Id4, differential expression between IHCs and OHCs was not detected by RNA-seq at P0, and it did not reach significance between KO and HET OHCs by RT–qPCR at E18.5. However, significance was achieved (P = 0.013) at E16.5, at which time differential expression was confirmed and visualized by RNAscope in situ hybridization (Fig. 4j). Hence, for Id4 the differential expression occurs transiently and very early.
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