Extended Data Fig. 6: Impaired spatial learning and memory after selective knockdown of YTHDF1 in the hippocampus of wild-type mice.
From: m6A facilitates hippocampus-dependent learning and memory through YTHDF1
a, Schematics of the AAV construct expressing YTHDF1 shRNA. b, Western blot and quantification of protein expression level of YTH proteins in N2A cells after YTHDF1-shRNA (RNAi) or control vector (Ctrl) transfection. For gel source data, see Supplementary Fig. 1. c, Spatial learning curves in the hidden-platform MWM training sessions for RNAi (red) and control (grey) mice. d–f, Spatial memory performances measured by quadrant time (%) (d) and number of platform crossings (e), and motor activities (f) of RNAi (red) and control (grey) mice in MWM probe tests. g, i, Contextual (g) and auditory (i) fear memories assessed 24 h after fear conditioning in RNAi and control mice. h, Anxiety level of mice assessed by open-arm durations in elevated-plus maze. P values, two-way repeated measures ANOVA with post hoc two-tailed t-test (c), two-way ANOVA with two-tailed t-test (comparison between group or to ‘Target’) (d), and two tailed t-test (b, e–i). Numbers in bars, numbers of biologically independent samples (b) and mice (d–i). Data shown as mean ± s.e.m.
