Extended Data Fig. 9: Effects of YTHDF1 on nascent protein synthesis in cultured hippocampal neurons in response to KCl stimulus.
From: m6A facilitates hippocampus-dependent learning and memory through YTHDF1
a, Additional representative images of nascent protein (Nascent-P) synthesis in cultured wild-type control and Ythdf1-KO hippocampal neurons before (sham) and 2 h after KCl depolarization, related to Fig. 4e, f. b, c, Representative images (b) and quantification (c) of Nascent-P in wild-type control and Ythdf1-KO hippocampal neurons before (sham) and 4 h after KCl depolarization. d, e, Representative images (d) and quantification (e) of Nascent-P in AAV-control and AAV-YTHDF1-RNAi treated hippocampal neurons before (sham), 2 h, and 4 h after KCl depolarization. Intensities of Nascent-P were normalized to that of wild-type control (c) or AAV-control (e) neurons under the sham condition. P values, two-tailed t-test (c, e). Numbers in bars, numbers of images/biologically independent samples. Data shown as mean ± s.e.m.
