Extended Data Fig. 5: Viral targeting in Ythdf1-KO mouse hippocampus and behavioural analyses of Ythdf1-KO mice injected with AAV virus. Related to Fig. 3.
From: m6A facilitates hippocampus-dependent learning and memory through YTHDF1
a, Representative fluorescence images of brain slices from rostral to caudal positions dissected from a mouse injected with AAV-YTHDF1 virus. Hoechst, blue; YTHDF1 co-expressed with mCherry, red. b, Representative images of virus expression (mCherry, red) and YTHDF1 immunostaining (green) in the mouse hippocampus after AAV-control or AAV-YTHDF1 infection. Hoechst, blue. c, YTHDF1 protein overexpression level indicated by immunofluorescent signal intensity in the CA1 and DG regions. d, Number of crossings over the previous platform location for Ythdf1-KO mice injected with AAV-YTHDF1 or AAV-control in MWM probe tests. e, Anxiety-like behaviour of the injected mice measured as open-arm durations in elevated-plus maze. f–h, Motor activities of the injected mice measured as total distance (f), number of moves (g), and average velocity (h) in the open-field test. P values, two tailed t-test (c-h). Numbers in bars, numbers of mice. Data shown as mean ± s.e.m.
