Extended Data Fig. 1: Phenotypic analysis of strains expressing HaloTag and Twin-Strep SprA fusion proteins.
From: Type 9 secretion system structures reveal a new protein transport mechanism
a, b, Immunoblot analysis of HaloTag-SprA (a) and Twin-Strep-SprA (b) expression in whole-cell lysates. Similar data were obtained for three biological repeats. c, Immunoblot detection of levels of the T9SS-dependent chitinase ChiA in culture supernatants. Similar data were obtained for three biological repeats. d, T9SS-dependent spreading (gliding) morphology of colonies on agar. Scale bar, 5 mm. Similar data were obtained for three biological repeats. e, Peptide mass spectrometry of the three highest molecular mass bands in Fig. 1d. Intensity values are normalized to the most abundant SprA peptide detected for each band. Peptide numbering is from the N terminus of the native SprA precursor sequence. For immunoblot source data, see Supplementary Fig. 1.
