Extended Data Fig. 8: TCR sharing and state transitions of CD8+ T cell clusters implicated by STARTRAC-tran indices.
From: Lineage tracking reveals dynamic relationships of T cells in colorectal cancer
a, Pie charts showing the fraction of shared clonotypes with CD8+ TEM cells within the other indicated clusters (left). P12 represents merged data of 12 patients with CRC. Bar plots showing the fraction of shared clonotypes of CD8+ TEM with other clusters within the CD8+ TEM. b, pSTARTRAC-tran indices of CD8+ TCM, TEMRA, TRM, IEL and TEX cells for each patient (depicted by dots). *P < 0.05, **P < 0.01, ***P < 0.001, Kruskal–Wallis test. c, Potential developmental trajectory of CD8+ T cells (n = 3,557, excluding MAIT cells) inferred by Monocle2 based on gene expressions. d, Frequency of shared clonotypes in CD8+ TEMRA cells with various TEM cell subsets in each patient (n = 12). e, Statistical analysis of tumour TEM shared TCRs with blood TEMRA and tumour TEX cells based on the number of clonotypes and clonal cells (related to Fig. 1h). ***P < 0.001, two-sided Fisher’s exact test. f, Clonotypes of tumour TEM cells crossing different clusters showing mutually exclusive TCR sharing of tumour TEM cells with blood TEMRA and tumour TEX cells. Each row represents an individual clonotype from one patient. *P < 0.05, **P < 0.01, ***P < 0.001, two-sided Fisher’s exact test (based on the number of clonal cells in each patient). Number of clonal cells analysed in each patient: P1212, n = 30; P1228, n = 27; P0411, n = 11; P0825, n = 10; P1012, n = 7; P0701, n = 9; P0123, n = 9; P0215, n = 17; P0309, n = 9; P0413, n = 2; P1207, n = 7; P0909, n = 2.
