Extended Data Fig. 5: Data from six individual brains for each brain from individuals with or without SAD represented as averages in Fig. 5, and variant cytotoxicity.
From: Somatic APP gene recombination in Alzheimer’s disease and normal neurons
a, d, Nuclei sorted from cortices of six individuals with SAD and six without were analysed by DISH16/17 (a) and DISH3/16 (d). Cumulative frequency distribution plots and average numbers of foci per nucleus show statistical significance (non-parametric Kruskal–Wallis test with Dunn’s correction for multiple comparisons) between all paired brain sets. Numbers above bars indicate number of nuclei analysed. NS, not significant. Error bars show s.e.m. b, c, e, f, Detailed P values for Fig. 5b (b), Fig. 5c (c), Fig. 5e (e) and Fig. 5f (f). g, APP-751, three coding and one non-coding APP variant in constructs containing haemagglutinin (HA) tags were transfected into HEK-293 cells. Cell lysates from all three coding variants and full-length APP-751 displayed protein products of the expected size by western blot. α-tubulin was used as a loading control. h, Three coding APP variants were transfected into SH-SY5Y cells individually and cell viability was measured by WST-1 seven days after transfection under serum-deprived conditions. Means of three independent experiments were analysed using ordinary one-way ANOVA with uncorrected Fisher’s LSD for multiple comparisons (*P = 0.0477, ****P < 0.0001).
