Extended Data Fig. 4: APP gencDNA and RNA variant formation in CHO cells.
From: Somatic APP gene recombination in Alzheimer’s disease and normal neurons
a, Time line of CHO cell experiments modified from Fig. 4a. After transfection and gencDNA induction, serum was added and CHO cell cultures were passaged for 7 days. Cells were harvested, and DNA and RNA were extracted for analyses. b, c, PCR of genomic DNA (b; gDNA) and RT–PCR with APP 1 and 18 primers (c; n = 2 independent experiments). Note that APP plasmid is no longer detected (compare to Fig. 4b). DNA breaks during cell proliferation might contribute to variant formation in cells without DNA damage (no H2O2). Reverse transcriptase inhibitor (RTi, AZT + ABC) treatment prevents formation of APP RNA variants, indicating the dependence of RNA variants on gencDNAs. d, Induced APP variants with IEJs observed in b, c.
