Extended Data Fig. 2: Mannose sensitivity is not associated with mannose uptake but with changes in cellular metabolism.
From: Mannose impairs tumour growth and enhances chemotherapy
a, Levels (expressed as peak area per microgram of proteins) of 2-deoxy-2-fluoro-d-mannose-phosphate (2-DFM-P) in RKO, SKOV3, SAOS-2 and U2OS-E1a (U2OS) cells after 6 h treatment with or without 2 mM 2-deoxy-2 fluoro-d-mannose. b–h, Relative levels of hexoses-6-phosphate (b), lactate (c), glyceraldehyde-3-phosphate (glyceraldehyde-3P) (d), phosphoenolpyruvate (e), malate (f), ribose-5-phosphate (ribose-5P) (g) and UDP-GlcNac (h) in SKOV3 and U2OS-E1a (U2OS) cells after 6 h treatment with or without 25 mM mannose in the culture medium (DMEM). Data in a are the average of three technical replicates and are representative of two independent experiments. In b–h, n = 2 independent experiments, each involving technical triplicates. All data are mean.
