Extended Data Fig. 1: Generation, genotyping and characterization of mouse C57BL/6N C2 Cdk1–TK/Cdk1 and Cdk1–TK/Cdk1–TK ES cells.
From: Linking a cell-division gene and a suicide gene to define and improve cell therapy safety
a, Summary of the targeting steps used to generate mouse C2 Cdk1–TK/Cdk1 and Cdk1–TK/Cdk1–TK ES cells. b, Southern blot genotyping with internal TK–mCherry probe. c, Southern blot genotyping with mouse Cdk1 genomic probe. d, In vitro GCV dose–response killing curve of mouse C2 CDK1–TK/CDK1 ES cells. Data are mean ± s.d., n = 3. e, Teratoma formation efficiency of mouse C2 Cdk1–TK-PB/Cdk1, Cdk1–TK/Cdk1 and Cdk1–TK/Cdk1–TK ES cells.
