Extended Data Fig. 1: Identification of enzymes involved in the SNO-CoA–SCoR system.
From: Metabolic reprogramming by the S-nitroso-CoA reductase system protects against kidney injury
a, b, Enzymatic mechanism by which the SNO-CoA–SCoR system regulates protein S-nitrosylation. a, Equilibrium between SNO-CoA and S-nitrosylated proteins. b, AKR1A1 (SCoR) mediates protein denitrosylation. c, AKR1A1 was purified to homogeneity using the indicated steps. AKR1A1 protein at each stage was calculated on the basis of activity in each eluate pool or original crude lysate. Image is representative of two independently performed experiments with similar results. d, e, Expression of iNOS, nNOS and eNOS in sham-treated versus injured kidneys of wild-type mice; injury induced by I/R. Images are representative of three independently performed experiments with similar results. For gel source data, see Supplementary Fig. 1. f, Expression of eNOS before and after AKI; normalized to GAPDH as in e (n = 8 mice per group). Results are presented as mean ± s.d. Two-tailed Student’s t-test was used to detect significance. g, Schema illustrating generation of Akr1a1−/− mice. h, PCR amplification of the Akr1a1 gene with genomic DNA isolated from the tails of Akr1a1+/+, heterozygous Akr1a1+/− and homozygous Akr1a1−/− mice. Image is representative of three independently performed experiments with similar results.
