Extended Data Fig. 5: Characterization of SNO-PKM2.
From: Metabolic reprogramming by the S-nitroso-CoA reductase system protects against kidney injury
a, Endogenous SNO level of PKM2 Cys-mutants in eNOS-overexpressing HEK-293 cells. Image is representative of two independently performed experiments with similar results. b, Mutation of C152 to alanine affects the SNO level of PKM2 in eNOS-overexpressing HEK-293 cells. Image is representative of two independently performed experiments with similar results. c, Quantification of expression of Myc–PKM2(WT), Myc–PKM2(C49A) and Myc–PKM2(C152A) in eNOS-overexpressing HEK-293 cells. Normalized to the expression of GAPDH (n = 5 independent experiments). d, Quantification of SNO-PKM2 in eNOS-overexpressing HEK-293 cells. SNO is normalized to PKM2 (input) (n = 5 independent experiments). e, Relative mRNA levels of Myc–PKM2(WT), Myc–PKM2(C49A) and Myc–PKM2(C152A) in eNOS-overexpressing HEK-293 cells (n = 3 independent experiments). Results presented as mean ± s.d. One-way ANOVA with Tukey post hoc test was used to detect significance.
